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structured illumination super resolution microscope  (Nikon)
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Nikon structured illumination super resolution microscope
( A ) Schematic depicting experimental design. ( B ) <t>Structured</t> <t>Illumination</t> super-resolution microscopy (SIM) image of dendritic spines on + /+, +/RC , and +/GS Drd1-Tomato expressing SPNs, labeled with antibodies to GluA1 (purple), and PSD95 (green). Open arrowheads, GluA1 nanodomains; arrowheads, PSD95 nanodomains. ( C ) Schematic diagram and object masks depicting GluA1, PSD95, and overlap nanodomains within a dendritic spine. Minimum distance between GluA1-PSD95 is measured from the closest edge of the two nanodomains, as shown. ( D, E ) Summary graphs and cumulative distribution of the minimum distance between GluA1 and PSD95 nanodomains. ( F, G ) Summary data and cumulative frequency for the overlap area of GluA1 and PSD95 nanodomains within dendritic spines. Asterisk in D and F reflect statistical significance for Tukey’s multiple comparison tests after one-way ANOVA, whereas asterisks in E and G show statistical significance for Bonferroni post-hoc comparisons after Kolmogorov-Smirnov tests. ( H ) Bar graphs showing the ratio of GluA1-PSD95 overlap area in E relative to PSD95 area, across genotypes. Data are represented as mean ± SEM. ( I-K ) Correlation plots of overlap in GluA1-PSD95 area versus PSD95 area for + /+, +/RC , and +/GS Drd1-Tomato expressing SPNs. *p<0.05, **p<0.01, ***p<0.001. Figure 2—source data 1. Raw data plotted in graphs in .
Structured Illumination Super Resolution Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) Schematic depicting experimental design. ( B ) Structured Illumination super-resolution microscopy (SIM) image of dendritic spines on + /+, +/RC , and +/GS Drd1-Tomato expressing SPNs, labeled with antibodies to GluA1 (purple), and PSD95 (green). Open arrowheads, GluA1 nanodomains; arrowheads, PSD95 nanodomains. ( C ) Schematic diagram and object masks depicting GluA1, PSD95, and overlap nanodomains within a dendritic spine. Minimum distance between GluA1-PSD95 is measured from the closest edge of the two nanodomains, as shown. ( D, E ) Summary graphs and cumulative distribution of the minimum distance between GluA1 and PSD95 nanodomains. ( F, G ) Summary data and cumulative frequency for the overlap area of GluA1 and PSD95 nanodomains within dendritic spines. Asterisk in D and F reflect statistical significance for Tukey’s multiple comparison tests after one-way ANOVA, whereas asterisks in E and G show statistical significance for Bonferroni post-hoc comparisons after Kolmogorov-Smirnov tests. ( H ) Bar graphs showing the ratio of GluA1-PSD95 overlap area in E relative to PSD95 area, across genotypes. Data are represented as mean ± SEM. ( I-K ) Correlation plots of overlap in GluA1-PSD95 area versus PSD95 area for + /+, +/RC , and +/GS Drd1-Tomato expressing SPNs. *p<0.05, **p<0.01, ***p<0.001. Figure 2—source data 1. Raw data plotted in graphs in .

Journal: eLife

Article Title: Pathway-specific dysregulation of striatal excitatory synapses by LRRK2 mutations

doi: 10.7554/eLife.58997

Figure Lengend Snippet: ( A ) Schematic depicting experimental design. ( B ) Structured Illumination super-resolution microscopy (SIM) image of dendritic spines on + /+, +/RC , and +/GS Drd1-Tomato expressing SPNs, labeled with antibodies to GluA1 (purple), and PSD95 (green). Open arrowheads, GluA1 nanodomains; arrowheads, PSD95 nanodomains. ( C ) Schematic diagram and object masks depicting GluA1, PSD95, and overlap nanodomains within a dendritic spine. Minimum distance between GluA1-PSD95 is measured from the closest edge of the two nanodomains, as shown. ( D, E ) Summary graphs and cumulative distribution of the minimum distance between GluA1 and PSD95 nanodomains. ( F, G ) Summary data and cumulative frequency for the overlap area of GluA1 and PSD95 nanodomains within dendritic spines. Asterisk in D and F reflect statistical significance for Tukey’s multiple comparison tests after one-way ANOVA, whereas asterisks in E and G show statistical significance for Bonferroni post-hoc comparisons after Kolmogorov-Smirnov tests. ( H ) Bar graphs showing the ratio of GluA1-PSD95 overlap area in E relative to PSD95 area, across genotypes. Data are represented as mean ± SEM. ( I-K ) Correlation plots of overlap in GluA1-PSD95 area versus PSD95 area for + /+, +/RC , and +/GS Drd1-Tomato expressing SPNs. *p<0.05, **p<0.01, ***p<0.001. Figure 2—source data 1. Raw data plotted in graphs in .

Article Snippet: Multichannel SIM images were obtained with a Nikon Structured Illumination super-resolution microscope using a 100x, 1.4 NA objective as previously described ( ).

Techniques: Super-Resolution Microscopy, Expressing, Labeling, Comparison